In each level, the goal is to reach the end point of the level, with the player scored on the number of LocoRocos found, the time to complete the level, and other factors. LocoRoco is divided into 5 worlds, each consisting of 8 levels. Kulche, a fun-loving yellow creature, defeats one of the Moja corp members, but the other Mojas finds out about this and chase after him. The planet wakes up to investigate the disaster striking the universe. Meanwhile, some of the Mojas begin draining the planet of its lifeforce.

Suddenly, a army of Mojas invade the planet, attempting to attack the LocoRocos. But then, a meteorite crashes into the universe. A group of joyful blob-like creatures, called the LocoRocos, live on a planet filled with natural beauty.